Leukocyte immunophenotyping: methodological aspects
نویسندگان
چکیده
منابع مشابه
Methodological aspects of anti-human leukocyte antigen antibody analysis in solid organ transplantation.
Donor human leukocyte antigen (HLA)-specific antibodies (DSA) play an important role in solid organ transplantation. Preexisting IgG isotype DSA are considered a risk factor for antibody mediated rejection, graft failure or graft loss. The post-transplant development of DSA depends on multiple factors including immunogenicity of mismatched antigens, HLA class II typing of the recipient, cytokin...
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This unit presents basic techniques for immunophenotyping by flow cytometry, direct using a conjugated monoclonal antibody and indirect using an unconjugated primary antibody followed by a conjugated secondary antibody. Combinations of these methods are described for two-, three-, and four-color staining. Analysis of data acquired from cells stained by these procedures is detailed. A procedure ...
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ژورنال
عنوان ژورنال: Critical Care
سال: 2010
ISSN: 1364-8535
DOI: 10.1186/cc8246